There has been some questions regarding the safety and efficacy of integrating spore-based probiotics (Bacillus) typically because many Americans are unfamiliar with them. Bacillus spores are and have been prescribed in europe and have been extensively studied in human clinical trials. 

Most often, we are familiar with lactobacillus sp. and bifidobacter sp. because they currently dominate the health industry. However, popularity does not equate to efficacy. When these organisms are supplemented orally they are generally destroyed by stomach acid and bile salts. This is just one of the many major benefits of spores, they survive through the gastric system and colonize the gastrointestinal tract.  

On this page I list scientific literature highlighting the use of these supplements. I have included the study, please feel free to read the full studies. 

This page will begin with reviews of the research and then each specific species and their documented use. Keep in mind, that much of the research reviews these strains and contain information within that can be perceived in different ways. 


The use of bacterial spore formers as probiotics

Bacillus probiotics

Bacillus probiotics

Bacillus indicus

The spore of Bacillus indicus produces 22 highly bioavailable forms of carotenoids such as astaxanthin, zeaxanthin, beta carotene and lutein at the site of absorption, the digestive tract. 

Carotenoids found in Bacillus.

Carotenoids present in halotolerant Bacillus spore formers.

Identification and the developmental formation of carotenoid pigments in the yellow-orange  Baccillus spore-formers.

Bacillus licheniformis

The highly effective biological preparation biosporin


Bacillus subtilis



Bacillus coagulans

In addition, spores have been shown to:

  • Facilitate production and absorption of critical nutrients such as carotenoids, quinols, vitamins, and enzymes.
  • Improve digestion of food and thus improved nutrient absorption.
  • Detoxification of the intestinal tract.
  • Reduce inflammation associated with Crohn's disease, IBS, ulcerative colitis. 
  • Improve growth of natural flora.